pET28a-eGFP-MinD
(Plasmid
#133622)
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PurposeExpression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5260
- Total vector size (bp) 6880
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEGFP-MinD
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SpeciesE. coli
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Insert Size (bp)1620
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Entrez GeneminD (a.k.a. b1175, ECK1163, minB)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-eGFP-MinD was a gift from Petra Schwille (Addgene plasmid # 133622 ; http://n2t.net/addgene:133622 ; RRID:Addgene_133622) -
For your References section:
Surface topology assisted alignment of Min protein waves. Zieske K, Schweizer J, Schwille P. FEBS Lett. 2014 Aug 1;588(15):2545-9. doi: 10.1016/j.febslet.2014.06.026. Epub 2014 Jun 14. 10.1016/j.febslet.2014.06.026 PubMed 24937143
Map uploaded by the depositor.
