pET28a-His-MinD_MinE
(Plasmid
#133621)
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PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133621 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5260
- Total vector size (bp) 6436
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMinD_MinE was amplified from plasmid pWM1255 which we received from William Margolin.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-His-MinD_MinE was a gift from Petra Schwille (Addgene plasmid # 133621 ; http://n2t.net/addgene:133621 ; RRID:Addgene_133621) -
For your References section:
Spatial regulators for bacterial cell division self-organize into surface waves in vitro. Loose M, Fischer-Friedrich E, Ries J, Kruse K, Schwille P. Science. 2008 May 9;320(5877):789-92. doi: 10.1126/science.1154413. 10.1126/science.1154413 PubMed 18467587
Map uploaded by the depositor.
