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Addgene

pMM766
(Plasmid #133603)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133603 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK001
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9 NLS Stop
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM766 was a gift from Megan McClean (Addgene plasmid # 133603 ; http://n2t.net/addgene:133603 ; RRID:Addgene_133603)

  • For your References section:

    Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization. Geller SH, Antwi EB, Di Ventura B, McClean MN. Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 10.1007/s12195-019-00598-9. eCollection 2019 Oct. 10.1007/s12195-019-00598-9 PubMed 31719930
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