pcDNA3-HA-VprBP (RARA)
(Plasmid
#133587)
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PurposeExpresses human VprBP RARA mutant in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5462
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVpr (HIV-1) binding protein
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Alt nameVprBP
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Alt nameKIAA0800
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Alt nameDCAF1
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SpeciesH. sapiens (human)
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Insert Size (bp)4542
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MutationRARA mutation of human VprBP defective in DDB1 binding
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GenBank IDNM_014703
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Entrez GeneDCAF1 (a.k.a. RIP, VPRBP)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The VprBP reading frame contains an L380P mismatch compared to NP_001336097.1 but functions as described in Nakagawa et al Mol Cell. 2015.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-HA-VprBP (RARA) was a gift from Yue Xiong (Addgene plasmid # 133587 ; http://n2t.net/addgene:133587 ; RRID:Addgene_133587) -
For your References section:
CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases. Nakagawa T, Lv L, Nakagawa M, Yu Y, Yu C, D'Alessio AC, Nakayama K, Fan HY, Chen X, Xiong Y. Mol Cell. 2015 Jan 22;57(2):247-60. doi: 10.1016/j.molcel.2014.12.002. Epub 2014 Dec 31. 10.1016/j.molcel.2014.12.002 PubMed 25557551