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Addgene

pCAGGS EFF-1::V5
(Plasmid #133584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4741
  • Total vector size (bp) 6797
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EFF-1::V5
  • Alt name
    pOA19
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2064
  • GenBank ID
    174334
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer pCAGGS Fw
  • 3′ sequencing primer pCAGGS Rv
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mohler WA, et al. Dec Cell 2002 Podbilewicz B, et al. Dec Cell 2006

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS EFF-1::V5 was a gift from Benjamin Podbilewicz (Addgene plasmid # 133584 ; http://n2t.net/addgene:133584 ; RRID:Addgene_133584)

  • For your References section:

    Conserved eukaryotic fusogens can fuse viral envelopes to cells. Avinoam O, Fridman K, Valansi C, Abutbul I, Zeev-Ben-Mordehai T, Maurer UE, Sapir A, Danino D, Grunewald K, White JM, Podbilewicz B. Science. 2011 Apr 29;332(6029):589-92. doi: 10.1126/science.1202333. Epub 2011 Mar 24. 10.1126/science.1202333 PubMed 21436398
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