pUC19_TTE1564_UUCG-loop
(Plasmid #133581)

• Purpose: T7-transcription template for a P2-deletion mutation of the Tte preQ1- riboswitch
• Depositing Lab: Nils Walter
• Publication: Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 5674991. doi: 10.1093/nar/gkz1166.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2700
• Total vector size (bp): 3710
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: P2-deletion mutant of Tte preQ1-riboswitch
• Species: Thermoanaerobacter tengcongensis MB4
• Insert Size (bp): 1030
• Mutation: replacement of riboswitch nucleotides U6 through C15 with UUCG tetraloop
• GenBank ID: 995666 995665
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: M13 Fwd
• 3′ sequencing primer: tgtggaattgtgagcgga

Resource Information

• Supplemental Documents:
  • pUC19_TTE1564_UUCG-loop.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Site-directed mutagenesis of the pUC19_TTE1564 plasmid (Addgene ID 61000), which carries the wild-type pseudoknot sequence, was performed to generate this P2-deletion mutant; the mutagenesis primer used was 5’-AAC AAA ATG CTC ACC TGG GTT CGC CCA GTT AAC AAA ACA AGG-3’.