PB CAG-R-bpA-3xSV40pA-R-td-sfGFP
(Plasmid
#133579)
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PurposeCAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133579 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCustom
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Vector typeMammalian Expression ; Dre/rox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTandem dimer superfolder GFP
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypiggyBac ITR's were synthesized from sequences in the NCBI database. CAG promoter is from pCAGGS obtained from BCCM/LMBP. bGH pA is from Neal Copeland. SV40 pA is from Clontech. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos. Backbone is from Stratagene.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
bGHpA-3xSV40pA stop cassette is from the ROSA26-CAG-loxP-EM7-Neo*-bpA-3xSV40pA-loxP-tauLacZ-bpA construct in Nam and Benezra, 2009 (https://www.ncbi.nlm.nih.gov/pubmed/19896442 Supplemental Data, Figure 3). When utilized in a reporter knocked in into a genomic locus, this stop cassette did not leak in vivo.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB CAG-R-bpA-3xSV40pA-R-td-sfGFP was a gift from Mario Capecchi (Addgene plasmid # 133579 ; http://n2t.net/addgene:133579 ; RRID:Addgene_133579)