PB CAG-F-bpA-3xSV40pA-F-td-sfGFP
(Plasmid #133573)

• Purpose: CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)
• Depositing Lab: Mario Capecchi
• Publication: piggyBac transposon plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Vector type: Mammalian Expression ; FLP/FRT

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tandem dimer superfolder GFP
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • ** 10 PB CAG-F-bpA-3pA-F-tdGFP-delXho1.gb
• A portion of this plasmid was derived from a plasmid made by: piggyBac ITR's were synthesized from sequences in the NCBI database. CAG promoter is from pCAGGS obtained from BCCM/LMBP. bGH pA is from Neal Copeland. SV40 pA is from Clontech. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos. Backbone is from Stratagene.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

bGHpA-3xSV40pA stop cassette is from the ROSA26-CAG-loxP-EM7-Neo*-bpA-3xSV40pA-loxP-tauLacZ-bpA construct in Nam and Benezra, 2009 (https://www.ncbi.nlm.nih.gov/pubmed/19896442 Supplemental Data, Figure 3). When utilized in a reporter knocked in into a genomic locus, this stop cassette did not leak in vivo.