pcDNA3-beta-HA-BRCA2
(Plasmid #133553)

• Purpose: Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF
• Depositing Lab: David Livingston
• Publication: Chen et al Mol Cell. 1998 Sep;2(3):317-28.

Backbone

• Vector backbone: pcDNA3-beta-HA
• Modifications to backbone: Restriction enzyme cloning of SalI BRCA2 fragment used to construct the plasmid.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BRCA2
• Species: H. sapiens (human)
• Entrez Gene: BRCA2 (a.k.a. BRCC2, BROVCA2, FACD, FAD, FAD1, FANCD, FANCD1, GLM3, PNCA2, XRCC11)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HMS clone ID number: HsCD00061621.

Contains the N372H and S599F variants in BRCA2