pOZC-PALB2
(Plasmid
#133552)
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PurposePALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOZ-FH-C
- Backbone size w/o insert (bp) 7900
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePALB2
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SpeciesH. sapiens (human)
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Entrez GenePALB2 (a.k.a. BROVCA5, FANCN, PNCA3)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: HsCD00061620.
Kozak sequence and ATG supplied by insert; stop codon present before Kozak to terminate upstream translation start.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOZC-PALB2 was a gift from David Livingston (Addgene plasmid # 133552 ; http://n2t.net/addgene:133552 ; RRID:Addgene_133552) -
For your References section:
Control of BRCA2 cellular and clinical functions by a nuclear partner, PALB2. Xia B, Sheng Q, Nakanishi K, Ohashi A, Wu J, Christ N, Liu X, Jasin M, Couch FJ, Livingston DM. Mol Cell. 2006 Jun 23. 22(6):719-29. 10.1016/j.molcel.2006.05.022 PubMed 16793542