pFA6a-mRFP1-kanMX6
(Plasmid
#133551)
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Purpose(Empty Backbone) Yeast fluorescent (RFP) vector with PTEF promoter.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133551 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a-mRFP1-kanMX6
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Backbone manufacturerO'Shea lab (HU/HMS)
- Backbone size (bp) 4846
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Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: EvNO00025949.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-mRFP1-kanMX6 was a gift from Won-Ki Huh & Erin O'Shea (Addgene plasmid # 133551 ; http://n2t.net/addgene:133551 ; RRID:Addgene_133551) -
For your References section:
Global analysis of protein localization in budding yeast. Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, O'Shea EK. Nature. 2003 Oct 16;425(6959):686-91. doi: 10.1038/nature02026. 10.1038/nature02026 PubMed 14562095
Map uploaded by the depositor.
