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Addgene

pBabe-Puro-REDD1-HA
(Plasmid #133550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe
  • Backbone size w/o insert (bp) 5086
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Regulated in development and DNA damage response 1 (REDD1)
  • Alt name
    REDD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    699
  • GenBank ID
    NM_019058.4
  • Entrez Gene
    DDIT4 (a.k.a. Dig2, REDD-1, REDD1)
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-Puro-REDD1-HA was a gift from James Brugarolas (Addgene plasmid # 133550 ; http://n2t.net/addgene:133550 ; RRID:Addgene_133550)

  • For your References section:

    Structural analysis and functional implications of the negative mTORC1 regulator REDD1. Vega-Rubin-de-Celis S, Abdallah Z, Kinch L, Grishin NV, Brugarolas J, Zhang X. Biochemistry. 2010 Mar 23;49(11):2491-501. doi: 10.1021/bi902135e. 10.1021/bi902135e PubMed 20166753
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