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Addgene

pY71-tet.I.-1
(Plasmid #133539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PY71
  • Backbone size w/o insert (bp) 1549
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Alt name
    T7 promoter
  • Alt name
    tetO
  • Species
    Synthetic
  • Insert Size (bp)
    1071
  • Mutation
    WT
  • Promoter T7
  • Tags / Fusion Proteins
    • TEV site (N terminal on backbone)
    • Strep tag (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTGTGATGCTCGTCAGGG
  • 3′ sequencing primer CTGCCTCGGTGAGTTTTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pY71-tet.I.-1 was a gift from Richard Murray (Addgene plasmid # 133539 ; http://n2t.net/addgene:133539 ; RRID:Addgene_133539)

  • For your References section:

    A method for cost-effective and rapid characterization of engineered T7-Based transcription factors by cell-free protein synthesis reveals insights into the regulation of T7 Rna polymerase-driven expression. McManus JB, Lux MW, Murray RM, Emanuel PA. Arch Biochem Biophys. 2019 Jul 18. pii: S0003-9861(19)30288-7. doi: 10.1016/j.abb.2019.07.010. 10.1016/j.abb.2019.07.010 PubMed 31326518
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