pY71-tet.I.11
(Plasmid
#133532)
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PurposesfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start site
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133532 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePY71
- Backbone size w/o insert (bp) 1549
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesfGFP
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Alt nameT7 promoter
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Alt nametetO
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SpeciesSynthetic
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Insert Size (bp)1071
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MutationWT
- Promoter T7
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Tags
/ Fusion Proteins
- TEV site (N terminal on backbone)
- Strep tag (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TTGTGATGCTCGTCAGGG
- 3′ sequencing primer CTGCCTCGGTGAGTTTTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pY71-tet.I.11 was a gift from Richard Murray (Addgene plasmid # 133532 ; http://n2t.net/addgene:133532 ; RRID:Addgene_133532) -
For your References section:
A method for cost-effective and rapid characterization of engineered T7-Based transcription factors by cell-free protein synthesis reveals insights into the regulation of T7 Rna polymerase-driven expression. McManus JB, Lux MW, Murray RM, Emanuel PA. Arch Biochem Biophys. 2019 Jul 18. pii: S0003-9861(19)30288-7. doi: 10.1016/j.abb.2019.07.010. 10.1016/j.abb.2019.07.010 PubMed 31326518