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Addgene

pAV0758
(Plasmid #133511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133511 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLys3BstZ17I(pAV0357)
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)
  • Species
    S. pombe (fission yeast)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a L368F mutation in Lys3. This mutation is not known to affect plasmid function.

Please see the Resource Information section for an annotated Genbank file.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV0758 was a gift from Sophie Martin (Addgene plasmid # 133511 ; http://n2t.net/addgene:133511 ; RRID:Addgene_133511)

  • For your References section:

    A toolbox of stable integration vectors in the fission yeast Schizosaccharomyces pombe. Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, Billault-Chaumartin I, Martin SG. J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 10.1242/jcs.240754. 10.1242/jcs.240754 PubMed 31801797
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