pUSti1
(Plasmid
#1335)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUGPD (pRS316 carrying GPD promoter, based on pBluescript)
- Backbone size w/o insert (bp) 5000
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSti1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2000
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Entrez GeneSTI1 (a.k.a. YOR027W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid maintained in DH5alpha, CEN and selectable URA3 marker. Full-length Sti1 gene, expressed at level comparable to the endogenous gene.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUSti1 was a gift from Susan Lindquist (Addgene plasmid # 1335 ; http://n2t.net/addgene:1335 ; RRID:Addgene_1335) -
For your References section:
In vivo analysis of the Hsp90 cochaperone Sti1 (p60). Chang HC, Nathan DF, Lindquist S. Mol Cell Biol 1997 Jan;17(1):318-25. PubMed 8972212