pXPR_124
(Plasmid
#133461)
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PurposeEF1a promoter expresses SpyoCas9 and P2A provides EGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133461 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV
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Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSpyoCas9
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Tag
/ Fusion Protein
- EGFP
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVectorBuilder, Inc.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXPR_124 was a gift from John Doench (Addgene plasmid # 133461 ; http://n2t.net/addgene:133461 ; RRID:Addgene_133461) -
For your References section:
Genetic screens in isogenic mammalian cell lines without single cell cloning. DeWeirdt PC, Sangree AK, Hanna RE, Sanson KR, Hegde M, Strand C, Persky NS, Doench JG. Nat Commun. 2020 Feb 6;11(1):752. doi: 10.1038/s41467-020-14620-6. 10.1038/s41467-020-14620-6 PubMed 32029722
