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Addgene

pET sfGFP(150TAG)
(Plasmid #133455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    744
  • Promoter T7
  • Tag / Fusion Protein
    • His

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CCGATTCTGGTGGAACTG
  • 3′ sequencing primer TAGGTCAGGGTGGTCAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET sfGFP(150TAG) was a gift from Yu-Hsuan Tsai (Addgene plasmid # 133455 ; http://n2t.net/addgene:133455 ; RRID:Addgene_133455)

  • For your References section:

    Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency. Williams TL, Iskandar DJ, Nodling AR, Tan Y, Luk LYP, Tsai YH. Amino Acids. 2021 Jan;53(1):89-96. doi: 10.1007/s00726-020-02927-z. Epub 2020 Dec 17. 10.1007/s00726-020-02927-z PubMed 33331978
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