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Addgene

SpyCatcher003-sfGFP
(Plasmid #133449)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133449 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ404
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5136
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyCatcher003-sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    1149
  • Promoter T5
  • Tags / Fusion Proteins
    • His6 (N terminal on backbone)
    • TEV site (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer lacOT5 (Gcgctcacaattccacaacggtttccc)
  • 3′ sequencing primer Term2 (CGAAAGGCTCAGTCGAAAGAC)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpyCatcher003-sfGFP was a gift from Mark Howarth (Addgene plasmid # 133449 ; http://n2t.net/addgene:133449 ; RRID:Addgene_133449)

  • For your References section:

    Approaching infinite affinity through engineering of peptide-protein interaction. Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, Hytonen VP, Howarth M. Proc Natl Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 10.1073/pnas.1909653116. 10.1073/pnas.1909653116 PubMed 31822621
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