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Purpose(Empty Backbone) Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133446 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeE.coli/S.aureus shuttle vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKOR1 was a gift from Taeok Bae & Olaf Schneewind (Addgene plasmid # 133446 ; http://n2t.net/addgene:133446 ; RRID:Addgene_133446) -
For your References section:
Allelic replacement in Staphylococcus aureus with inducible counter-selection. Bae T, Schneewind O. Plasmid. 2006 Jan;55(1):58-63. doi: 10.1016/j.plasmid.2005.05.005. Epub 2005 Jul 26. 10.1016/j.plasmid.2005.05.005 PubMed 16051359