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Addgene

NW30
(Plasmid #133444)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133444 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NW30
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1758

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMS clone ID number: HsCD00483426.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NW30 was a gift from Gerhard Wagner (Addgene plasmid # 133444 ; http://n2t.net/addgene:133444 ; RRID:Addgene_133444)

  • For your References section:

    Covalently circularized nanodiscs for studying membrane proteins and viral entry. Nasr ML, Baptista D, Strauss M, Sun ZJ, Grigoriu S, Huser S, Pluckthun A, Hagn F, Walz T, Hogle JM, Wagner G. Nat Methods. 2017 Jan;14(1):49-52. doi: 10.1038/nmeth.4079. Epub 2016 Nov 21. 10.1038/nmeth.4079 PubMed 27869813
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