KE-009
(Plasmid
#133441)
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PurposeHuman elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133441 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21 elF4H
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEIF4H
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SpeciesH. sapiens (human)
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Insert Size (bp)903
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Entrez GeneEIF4H (a.k.a. WBSCR1, WSCR1, eIF-4H)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: HsCD00483423.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KE-009 was a gift from Gerhard Wagner (Addgene plasmid # 133441 ; http://n2t.net/addgene:133441 ; RRID:Addgene_133441)