pET30-GBFusion1
(Plasmid
#133438)
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PurposeStreptococcus GB1 domain in vector for bacterial expression.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133438 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET30a(+)
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGB1 domain
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SpeciesStreptococcus
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MutationThis fragment encodes only aa 1 to 56.
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: StCD00082679.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET30-GBFusion1 was a gift from Gerhard Wagner (Addgene plasmid # 133438 ; http://n2t.net/addgene:133438 ; RRID:Addgene_133438) -
For your References section:
A solubility-enhancement tag (SET) for NMR studies of poorly behaving proteins. Zhou P, Lugovskoy AA, Wagner G. J Biomol NMR. 2001 May . 20(1):11-4. 10.1023/A:1011258906244 PubMed 11430750