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Addgene

pET30-GBFusion1
(Plasmid #133438)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133438 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET30a(+)
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GB1 domain
  • Species
    Streptococcus
  • Mutation
    This fragment encodes only aa 1 to 56.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMS clone ID number: StCD00082679.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-GBFusion1 was a gift from Gerhard Wagner (Addgene plasmid # 133438 ; http://n2t.net/addgene:133438 ; RRID:Addgene_133438)
  • For your References section:

    A solubility-enhancement tag (SET) for NMR studies of poorly behaving proteins. Zhou P, Lugovskoy AA, Wagner G. J Biomol NMR. 2001 May . 20(1):11-4. 10.1023/A:1011258906244 PubMed 11430750