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pPICZaa-STE2-cohis
(Plasmid #133433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133433 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPICZalpha
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STE2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1290
  • Mutation
    C59S/C252A, Codon optimized for P. pastoris, and unique restriction sites added to gene
  • Entrez Gene
    STE2 (a.k.a. YFL026W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMS clone ID number: ScCD00063799.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPICZaa-STE2-cohis was a gift from Gerhard Wagner (Addgene plasmid # 133433 ; http://n2t.net/addgene:133433 ; RRID:Addgene_133433)
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