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Addgene

pET-24a-STE2
(Plasmid #133430)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133430 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET24a
  • Backbone size w/o insert (bp) 5310
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STE2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    990
  • Mutation
    Both native Cys (C59S/C252A) have been mutated. Synthetic gene with engineered unique restriction sites.
  • Entrez Gene
    STE2 (a.k.a. YFL026W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMS clone ID number: ScCD00063796.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-24a-STE2 was a gift from Gerhard Wagner (Addgene plasmid # 133430 ; http://n2t.net/addgene:133430 ; RRID:Addgene_133430)