pETM30-ARC105
(Plasmid
#133426)
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PurposeHuman ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET30 (modified)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePCQAP
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SpeciesH. sapiens (human)
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Insert Size (bp)911
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MutationContains amino acids 5-78 fused to C-terminal of GST
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Entrez GeneMED15 (a.k.a. ARC105, CAG7A, CTG7A, PCQAP, TIG-1, TIG1, TNRC7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: HsCD00063791.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETM30-ARC105 was a gift from Gerhard Wagner (Addgene plasmid # 133426 ; http://n2t.net/addgene:133426 ; RRID:Addgene_133426) -
For your References section:
An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis. Yang F, Vought BW, Satterlee JS, Walker AK, Jim Sun ZY, Watts JL, DeBeaumont R, Saito RM, Hyberts SG, Yang S, Macol C, Iyer L, Tjian R, van den Heuvel S, Hart AC, Wagner G, Naar AM. Nature. 2006 Aug 10;442(7103):700-4. doi: 10.1038/nature04942. Epub 2006 Jun 21. 10.1038/nature04942 PubMed 16799563
