pYES2-STE2-1D4
(Plasmid
#133424)
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PurposeMutant yeast STE2 coding sequence in vector for expression in yeast S. cerevisiae, with Gal1 promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133424 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYES2
- Backbone size w/o insert (bp) 5900
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSTE2
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1040
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MutationC59S, C252A
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Entrez GeneSTE2 (a.k.a. YFL026W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: ScCD00063806.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYES2-STE2-1D4 was a gift from Gerhard Wagner (Addgene plasmid # 133424 ; http://n2t.net/addgene:133424 ; RRID:Addgene_133424)