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pst39-NST-clone14
(Plasmid #133421)

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  • Purpose
    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).
  • Depositing Lab
    Gerhard Wagner
  • Publication
    Wagner Lab plasmids (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST39
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NIP1/SUI1/TIF5
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    ORF for Nip1 encodes aa 4-156 (not complete ORF), ORF for Tif5 (B6) encodes aa 241-405 (not complete ORF)
  • Entrez Gene
    NIP1 (a.k.a. YMR309C)
  • Entrez Gene
    TIF5 (a.k.a. YPR041W, SUI5)
  • Entrez Gene
    SUI1 (a.k.a. YNL244C, MOF2, RFR1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMS clone ID number: ScCD00061648.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pst39-NST-clone14 was a gift from Gerhard Wagner (Addgene plasmid # 133421 ; http://n2t.net/addgene:133421 ; RRID:Addgene_133421)
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