pAAV2-GCaMP6f-P2A-H2B-PAmCherry
(Plasmid #133419)

• Purpose: Fluorescent reporter for calcium imaging and photoactivation
• Depositing Lab: Tim Holy
• Publication: Lee et al Science. 2019 Dec 13;366(6471):1384-1389. doi: 10.1126/science.aax8055.

Backbone

• Vector backbone: pAAV2
• Backbone size w/o insert (bp): 4625
• Total vector size (bp): 7166
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCaMP6f-P2A-H2B-PAmCherry
• Species: H. sapiens (human)
• Insert Size (bp): 2541
• GenBank ID: NM_021058.3
• Entrez Gene: H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
• Promoter: CAG

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: EBV Reverse

Resource Information

• Supplemental Documents:
  • pTR-UF-12-new-gcamp6f-p2a-h2b-pamcherry.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pAAV2 : The Hope Center Viral Vectors Core at Washington University in St. Louis
GCaMP6f :pGP-CMV-GCaMP6f was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 40755 ; http://n2t.net/addgene:40755 ; RRID:Addgene_40755)
PAmCherry: pPAmCherry1-C1 was a gift from Vladislav Verkhusha (Addgene plasmid # 31929 ; http://n2t.net/addgene:31929 ; RRID:Addgene_31929)
H2B: H2B-GFP was a gift from Geoff Wahl (Addgene plasmid # 11680 ; http://n2t.net/addgene:11680 ; RRID:Addgene_11680)