pAAV2-GCaMP6f-P2A-Vmn1r237
(Plasmid #133417)

• Purpose: Fluorescent calcium reporter and mouse vomeronasal 1 receptor 237
• Depositing Lab: Tim Holy
• Publication: Lee et al Science. 2019 Dec 13;366(6471):1384-1389. doi: 10.1126/science.aax8055.

Backbone

• Vector backbone: pAAV2
• Backbone size w/o insert (bp): 4619
• Total vector size (bp): 6923
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCaMP6f-P2A-vmn1r237
• Species: M. musculus (mouse)
• Insert Size (bp): 2304
• GenBank ID: NM_134200.1
• Entrez Gene: Vmn1r237 (a.k.a. V1rf3, Vfrf3)
• Promoter: CAG

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: EBV Reverse

Resource Information

• Supplemental Documents:
  • pTR-UF-12-new-gcamp6f-p2a-vmn1r237.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pAAV2 : The Hope Center Viral Vectors Core at Washington University in St. Louis
GCaMP6f :pGP-CMV-GCaMP6f was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 40755 ; http://n2t.net/addgene:40755 ; RRID:Addgene_40755)