pAAV2-GCaMP6f-P2A-Vmn1r85
(Plasmid
#133414)
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PurposeFluorescent calcium reporter and mouse vomeronasal 1 receptor 85
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133414 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV2
- Backbone size w/o insert (bp) 4619
- Total vector size (bp) 6980
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCaMP6f-P2A-vmn1r78
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SpeciesM. musculus (mouse)
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Insert Size (bp)2361
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GenBank IDNM_145847.1
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Entrez GeneVmn1r78 (a.k.a. V1rg7)
- Promoter CAG
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer EBV Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pAAV2 : The Hope Center Viral Vectors Core at Washington University in St. Louis
GCaMP6f :pGP-CMV-GCaMP6f was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 40755 ; http://n2t.net/addgene:40755 ; RRID:Addgene_40755)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV2-GCaMP6f-P2A-Vmn1r85 was a gift from Tim Holy (Addgene plasmid # 133414 ; http://n2t.net/addgene:133414 ; RRID:Addgene_133414) -
For your References section:
Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types. Lee D, Kume M, Holy TE. Science. 2019 Dec 13;366(6471):1384-1389. doi: 10.1126/science.aax8055. 10.1126/science.aax8055 PubMed 31831669