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pAAV2-GCaMP6f-P2A-PAmCherry
(Plasmid #133412)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133412 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV2
  • Backbone size w/o insert (bp) 4632
  • Total vector size (bp) 6777
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP6f-P2A-PAmCherry
  • Insert Size (bp)
    2145
  • Promoter CAG

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pAAV2 : The Hope Center Viral Vectors Core at Washington University in St. Louis
GCaMP6f :pGP-CMV-GCaMP6f was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 40755 ; http://n2t.net/addgene:40755 ; RRID:Addgene_40755)
PAmCherry: pPAmCherry1-C1 was a gift from Vladislav Verkhusha (Addgene plasmid # 31929 ; http://n2t.net/addgene:31929 ; RRID:Addgene_31929)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV2-GCaMP6f-P2A-PAmCherry was a gift from Tim Holy (Addgene plasmid # 133412 ; http://n2t.net/addgene:133412 ; RRID:Addgene_133412)

  • For your References section:

    Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types. Lee D, Kume M, Holy TE. Science. 2019 Dec 13;366(6471):1384-1389. doi: 10.1126/science.aax8055. 10.1126/science.aax8055 PubMed 31831669
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