GR A458T
(Plasmid
#133410)
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Purposeoverexpression of a mutant human glucocorticoid receptor in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNR3C1
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SpeciesH. sapiens (human)
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MutationA458T
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Entrez GeneNR3C1 (a.k.a. GCCR, GCR, GCRST, GR, GRL)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GR A458T was a gift from Andrew Cato (Addgene plasmid # 133410 ; http://n2t.net/addgene:133410 ; RRID:Addgene_133410) -
For your References section:
A distinct modulating domain in glucocorticoid receptor monomers in the repression of activity of the transcription factor AP-1. Heck S, Kullmann M, Gast A, Ponta H, Rahmsdorf HJ, Herrlich P, Cato AC. EMBO J. 1994 Sep 1;13(17):4087-95. PubMed 8076604