BII-CMV-AfmW3A
(Plasmid #133402)

• Purpose: Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells
• Depositing Lab: Blair Madison
• Publication: Fischer et al Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 10.1152/ajpgi.00058.2022.

Backbone

• Vector backbone: N/A
• Backbone manufacturer: Madison Lab
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Afamin and Wnt3a
• Species: M. musculus (mouse)
• Mutation: N/A
• Entrez Gene: Afm (a.k.a. Alf, MGC118711, alpha-Alb)
• Entrez Gene: Wnt3a (a.k.a. Wnt-3a, vt)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • BII-CMV-AfmW3a.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2020.10.14.339531 for bioRxiv preprint.