BII-ChPtW-iRFP670
(Plasmid
#133397)
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PurposePiggybac vector for constitutive expression of iRFP670-NLS
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133397 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBII-ChPtW
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Backbone manufacturerMadison Lab
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiRFP-670-NLS
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SpeciesOther
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MutationN/A
- Promoter CMV enhancer and hEf1a (CpG free)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BII-ChPtW-iRFP670 was a gift from Blair Madison (Addgene plasmid # 133397 ; http://n2t.net/addgene:133397 ; RRID:Addgene_133397)