BII-TOP-tdT
(Plasmid
#133395)
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PurposePiggybac vector for canonical Wnt signaling reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Backbone manufacturerMadison Lab
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametdTomato-NLS
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SpeciesOther
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MutationN/A
- Promoter SuperTOPFlash
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BII-TOP-tdT was a gift from Blair Madison (Addgene plasmid # 133395 ; http://n2t.net/addgene:133395 ; RRID:Addgene_133395) -
For your References section:
The Zinc Finger Transcription Factor PLAGL2 Enhances Stem Cell Fate and Activates Expression of ASCL2 in Intestinal Epithelial Cells. Strubberg AM, Veronese Paniagua DA, Zhao T, Dublin L, Pritchard T, Bayguinov PO, Fitzpatrick JAJ, Madison BB. Stem Cell Reports. 2018 Aug 14;11(2):410-424. doi: 10.1016/j.stemcr.2018.06.009. Epub 2018 Jul 12. 10.1016/j.stemcr.2018.06.009 PubMed 30017821