Skip to main content
Addgene

BII-BCA-Prom-ASCL2
(Plasmid #133394)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    BII-BCA-Prom
  • Backbone manufacturer
    Madison Lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    destabilized tdTomato-NLS and destabilized NLS-GFP
  • Species
    H. sapiens (human)
  • Mutation
    N/A
  • Promoter CMV enhancer and hEf1a (CpG free) drives expression of GFP and human ASCL2 promoter drives expression of tdTomato

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BII-BCA-Prom-ASCL2 was a gift from Blair Madison (Addgene plasmid # 133394 ; http://n2t.net/addgene:133394 ; RRID:Addgene_133394)

  • For your References section:

    The Zinc Finger Transcription Factor PLAGL2 Enhances Stem Cell Fate and Activates Expression of ASCL2 in Intestinal Epithelial Cells. Strubberg AM, Veronese Paniagua DA, Zhao T, Dublin L, Pritchard T, Bayguinov PO, Fitzpatrick JAJ, Madison BB. Stem Cell Reports. 2018 Aug 14;11(2):410-424. doi: 10.1016/j.stemcr.2018.06.009. Epub 2018 Jul 12. 10.1016/j.stemcr.2018.06.009 PubMed 30017821
Related items:
Commonly requested with: