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Addgene

pBabe puro pI3K p110 CAAX
(Plasmid #13339)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13339 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pI3K p110 CAAX
  • Alt name
    pI3K
  • Alt name
    p110
  • Alt name
    PIK3CA
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    3200
  • GenBank ID
    NM_174574
  • Entrez Gene
    PIK3CA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pBabe 5'
  • 3′ sequencing primer pBabe 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasma membrane-targeted, constitutively activated PI3K p110. Please note that the myc tag is NONFUNCTIONAL.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe puro pI3K p110 CAAX was a gift from Channing Der (Addgene plasmid # 13339 ; http://n2t.net/addgene:13339 ; RRID:Addgene_13339)

  • For your References section:

    Oncogenic Ras blocks anoikis by activation of a novel effector pathway independent of phosphatidylinositol 3-kinase. McFall A, Ulku A, Lambert QT, Kusa A, Rogers-Graham K, Der CJ. Mol Cell Biol. 2001 Aug . 21(16):5488-99. 10.1128/MCB.21.16.5488-5499.2001 PubMed 11463831
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