Raf-1 GST RBD 1-149
(Plasmid #13338)

• Depositing Lab: Channing Der
• Publication: Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-12.

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: Amersham Pharmacia
• Backbone size w/o insert (bp): 4950
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Raf-1
• Alt name: Raf1
• Species: H. sapiens (human)
• Insert Size (bp): 450
• Entrez Gene: RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Resource Information

• Articles Citing this Plasmid:
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pGEX-2T bacterial expression vector encoding GST followed by N-terminal residues 2-149 of human Raf-1. Contains the Ras-binding domain that binds preferentially to activated Ras-GTP. Used for determination of Ras-GTP formation in mammalian cell lysates.