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BII-gR-PtW-eSpCas9
(Plasmid #133357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    BII-gR-PtW-EspCas9
  • Backbone manufacturer
    Madison Lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eSpCas9
  • gRNA/shRNA sequence
    GGAGACGCTGACCCGTCTCT
  • Species
    Staphalococcus pyogenes
  • Mutation
    eSpCas9 mutations
  • Promoter CMV enhancer and hEf1a (CpG free)
  • Tag / Fusion Protein
    • HA-tagged eSpCas9 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2020.10.14.339531 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BII-gR-PtW-eSpCas9 was a gift from Blair Madison (Addgene plasmid # 133357 ; http://n2t.net/addgene:133357 ; RRID:Addgene_133357)

  • For your References section:

    The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and a Wnt-independent mechanism in colorectal cancer. Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin DC, Madison BB. Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 10.1152/ajpgi.00058.2022. 10.1152/ajpgi.00058.2022 PubMed 37310750
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