BII-gR-PnTW
(Plasmid #133356)

• Purpose: Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
• Depositing Lab: Blair Madison
• Publication: Madison Lab plasmids (unpublished)

Backbone

• Vector backbone: BII-gR-PnTW
• Backbone manufacturer: Madison Lab
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tdTomato-NLS
• gRNA/shRNA sequence: GGAGACGCTGACCCGTCTCT
• Species: Other
• Mutation: N/A
• Promoter: CMV enhancer and hEf1a (CpG free)
• Tag / Fusion Protein:
  • HA-tagged tdTomato (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • BII-gR-PnTW.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry