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Addgene

Major Sat-BUB1
(Plasmid #133350)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133350 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTALYM3B15
  • Backbone manufacturer
    Maria-Elena Torres-Padilla
  • Total vector size (bp) 9067
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BUB1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1161
  • Mutation
    deleted amino acids 1-671
  • Entrez Gene
    Bub1 (a.k.a. AL022991, Bub1a, C80208, D2Xrf8, D2Xrf87)
  • Tag / Fusion Protein
    • mClover (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGGACGAGCTGTACAAGGGTGGTGAGGCTTTTAAATGCACAGG
  • 3′ sequencing primer GTACCTTAAGCCTAGGTTATTTTCTTGAACGCTTAT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Major Sat-BUB1 was a gift from Michael Lampson (Addgene plasmid # 133350 ; http://n2t.net/addgene:133350 ; RRID:Addgene_133350)

  • For your References section:

    Molecular Strategies of Meiotic Cheating by Selfish Centromeres. Akera T, Trimm E, Lampson MA. Cell. 2019 Aug 22;178(5):1132-1144.e10. doi: 10.1016/j.cell.2019.07.001. Epub 2019 Aug 8. 10.1016/j.cell.2019.07.001 PubMed 31402175
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