pDR119
(Plasmid #13332)

• Depositing Lab: Martin Gellert
• Publication: Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DNA ligase IV
• Species: H. sapiens (human)
• Entrez Gene: LIG4 (a.k.a. LIG4S)
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full length DNA ligase IV was fused to a polyhistidine tag at the C-terminus and subcloned into pET28a.