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Addgene

pBMM42
(Plasmid #13331)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13331 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XRCC4
  • Species
    H. sapiens (human)
  • Entrez Gene
    XRCC4 (a.k.a. SSMED, hXRCC4)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The human XRCC4 open reading frame was cloned and polyhistidine tagged at the 3' end by Pfu PCR from a human thymus cDNA pool (Clontech), and subcloned into pET28a.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMM42 was a gift from Martin Gellert (Addgene plasmid # 13331 ; http://n2t.net/addgene:13331 ; RRID:Addgene_13331)
  • For your References section:

    DNA binding of Xrcc4 protein is associated with V(D)J recombination but not with stimulation of DNA ligase IV activity. Modesti M, Hesse JE, Gellert M. EMBO J. 1999 Apr 1. 18(7):2008-18. 10.1093/emboj/18.7.2008 PubMed 10202163