OA-1050J (GFP)
(Plasmid
#133304)
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Purposeexpress arrays of gRNA targeting GFP under dU6-3 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133304 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonewhite+attB+GFP
- Backbone size w/o insert (bp) 9351
- Total vector size (bp) 9658
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Vector typeInsect Expression
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Selectable markersmini-white, GFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP gRNA array
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gRNA/shRNA sequenceCAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAACGCAGATGAACTTCAGGGTCAGCTTGCCGTACAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAACTCATGTGGTCGGGGTAGCGGCTGAAGCACTCAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAACACTCCAGCTTGTGCCCCAGGATGTTGCCGTCAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAACTCACCTTGATGCCGTTCTTCTGCTTGTCGGCAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAAC
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SpeciesSynthetic
- Promoter U6-3
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OA-1050J (GFP) was a gift from Omar Akbari (Addgene plasmid # 133304 ; http://n2t.net/addgene:133304 ; RRID:Addgene_133304) -
For your References section:
Programmable RNA Targeting Using CasRx in Flies. Buchman AB, Brogan DJ, Sun R, Yang T, Hsu PD, Akbari OS. CRISPR J. 2020 Jun;3(3):164-176. doi: 10.1089/crispr.2020.0018. 10.1089/crispr.2020.0018 PubMed 32584145
