pOz-humBirA-GFP
(Plasmid #133283)

• Purpose: Expression of BirA-GFP fusions in mammalian cells
• Depositing Lab: Arman Kulyyassov
• Publication: Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: biom10010142. doi: 10.3390/biom10010142.

Backbone

• Vector backbone: pOzFHHN
• Backbone size w/o insert (bp): 8901
• Total vector size (bp): 9618
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP
• Alt name: eGFP
• Species: Aequorea victoria
• Insert Size (bp): 717
• GenBank ID: KJ541667
• Promoter: MoMuLV
• Tag / Fusion Protein:
  • Humanized wild type biotin ligase BirA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CTCTGGCTAACTAGAGAAC
• 3′ sequencing primer: AGACGGCAATATGGTGGA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Please note that the plasmid map and some versions of SnapGene incorrectly identify BirA as a mutant. We have verified that this sequence is the WT BirA gene. Addgene is working to fix this issue.

1. Kulyyassov, A. et al. PUB-MS: A Mass Spectrometry-based Method to Monitor Protein-Protein Proximity in vivo. Journal of Proteome Research10, 4416-4427, doi:10.1021/pr200189p (2011).
2. Shoaib, M. et al. PUB-NChIP-"in vivo biotinylation" approach to study chromatin in proximity to a protein of interest. Genome Research23, 331-340, doi:10.1101/gr.134874.111 (2013).