pJH548
(Plasmid #13328)

• Depositing Lab: Martin Gellert
• Publication: Sadofsky et al Nucleic Acids Res. 1993 Dec 11. 21(24):5644-50.

Backbone

• Vector backbone: pCDM8
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4356
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Growth instructions: Grow in SupF strain. The supF tRNA suppressor gene is used for selection in E. coli.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RAG-1
• Alt name: RAG1
• Species: M. musculus (mouse)
• Entrez Gene: Rag1 (a.k.a. Rag-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cDNA was synthesized by reverse transcription of total RNA obtained from cell line 22D6, an Abelson murine leukemia virus transformed pre-B cell line. RAG-1 cDNA was then amplified by PCR, using specific primers designed also to add restriction enzyme recognition sites adjacent to the largest open reading frame for subsequent cloning. PCR products were subcloned into the pCDM8 vector.