pIRES:mTREK-1(G137I)
(Plasmid #133274)

• Purpose: Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag
• Depositing Lab: Dan Minor
• Publication: Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. doi: 10.1038/nature22988. Epub 2017 Jul 10.

Backbone

• Vector backbone: pIRES2-EGFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Potassium channel subfamily K member 2 (KCNK2, TREK-1)
• Species: M. musculus (mouse)
• Mutation: G137I
• Entrez Gene: Kcnk2 (a.k.a. A430027H14Rik, TREK-1)
• Promoter: CMVd2
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV F
• 3′ sequencing primer: CMV R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains the CMVd2 promoter. The plasmid works as intended, and expression levels are not affected.