pPICZ:mTREK-1_cryst
(Plasmid #133269)

• Purpose: P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP
• Depositing Lab: Dan Minor
• Publication: Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. doi: 10.1038/nature22988. Epub 2017 Jul 10.

Backbone

• Vector backbone: pPICZ
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Potassium channel subfamily K member 2 (KCNK2, TREK-1)
• Species: M. musculus (mouse)
• Mutation: aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A92P, N95S, S96D, T97Q, N119A, S300A, E306A
• Entrez Gene: Kcnk2 (a.k.a. A430027H14Rik, TREK-1)
• Tag / Fusion Protein:
  • SNS- 3C_cleavage_site-TAAA-GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: AOX5
• 3′ sequencing primer: AOX3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry