pMXs_FLAG-GGC1
(Plasmid #133258)

• Purpose: Express FLAG-tagged GGC1 in mammalian cells
• Depositing Lab: David Sabatini
• Publication: Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi: 10.1126/sciadv.abe5310. Print 2020 Oct.

Backbone

• Vector backbone: pMXs
• Backbone manufacturer: Cell Biolabs, Inc.
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GGC1
• Alt name: YDL198C
• Species: S. cerevisiae (budding yeast)
• Mutation: codon-optimized for expression in human cells
• Entrez Gene: GGC1 (a.k.a. YDL198C, SHM1, YHM1)
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: GTGGACCATCCTCTAG
• 3′ sequencing primer: GACGGCAATATGGTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry